Anti-metastatic profiles of Boesenbergia pandurata towards MCF-7/HER2 cells
Penulis/Author
HILYATUL FADLIYAH (1); NINDYA BUDIANA PUTRI (2); ZIANA WALIDAH (3); IKA PUTRI NURHAYATI (4); Dr. apt. Muthi' Ikawati, M.Sc. (5); Prof. Dr. apt. Edy Meiyanto, M.Si. (6)
Tanggal/Date
2018
Kata Kunci/Keyword
Abstrak/Abstract
The development of breast cancer at an advanced stage is signed with metastatic phenomenon, triggering the high mortality, mainly for Human Epidermal Growth Factor Receptor (HER)2 positive cancers. Boesenbergia pandurata is well known as medicinal plant possessing anticancer potential due to the cytototoxic and antimetastatic characteristic of its active compound. The aim of this study is to observe the inhibitory effect of Boesenbergia pandurata ethanolic extract (BPEE) in combination with doxorubicin on migration of MCF-7/HER2 cells. The BPEE was prepared by 96% ethanol maceration. Under MTT assay, BPEE decreased the cells viability with IC50 value of 23±3.9 ?g/mL. Lamellipodia and wound healing assay analysis showed that 5 ?g/ mL BPPE and its combination with 10 nM doxorubicin inhibited cells migration after 48 hours observation, while gelatin zymography analysis showed that this combination did not affect the expression of Matrix Metalloproteinase (MMP)2 and MMP9, but single treatment of 5 ?g/mL BPEE caused lower expression of both MMPs. The combination of 5 ?g/mL BPPE and 10 nM doxorubicin inhibited the cells migration but not affect to the cells viability. Thus, BPEE is potential to be developed as an antimetastatic agent. The mechanism underlying the migratory inhibition effect needs to be explored further.
Rumpun Ilmu
Analisis Farmasi dan Kimia Medisinal
Bahasa Asli/Original Language
English
Level
Nasional
Status
Dokumen Karya
No
Judul
Tipe Dokumen
Aksi
1
2018-Fadliyah et al-Boesenrbergia on MCF7:HER2.pdf
