Anti-metastatic Effect of Curcumin on Triple Negative Breast Cancer
Penulis/Author
drh. Retno Murwanti, M.P., Ph.D. (1); Prof. Dr.rer.nat. apt. Adam Hermawan, S.Farm., M.Sc. (2); Dr. B.S. Ari Sudarmanto, M.Si. (3)
Tanggal/Date
22 2019
Kata Kunci/Keyword
Abstrak/Abstract
Triple negative breast cancer (TNBC) accounts for 10–20% of all cases of breast carcinoma. People who have TNBC are also more likely to develop metastasis. Metastasis refers to a secondary cancer forming in a different part of the body. Metastases are often more dangerous than the primary tumor and they are responsible for 90% of all cancer deaths. The purpose of this study is to explore the anti-metastatic activities of curcumin on 4T1 triple negative breast cancer cells. Gelatin zymography assay was carried out to determine inhibition of MMP-9 activity, while MMP-9 mRNA expression was analyzed using RT-PCR. The testing of anti metastatsis using the gelatin zymography demonstrated that curcumin significantly inhibited the activity of MMP-9 on 4T1 cells compare to the control. The MMP-9 mRNA expression was not lowered upon curcumin treatment. This results indicates that inhibition of metastasis in 4T1 is not due to inhibition of mRNA expression. So, further studies are needed to see the mechanism of curcumin to inhibit metastasis.
