| Abstrak/Abstract |
Andrographolide has been shown to have a pharmacological effect as an antidiabetic. Nevertheless, the comprehensive mechanism of action has not yet been determined. Andrographolide is one of the primary components of the sambiloto herb (Andrographis paniculata (Burm.f.) Nees), which a simple isolation process can obtain with high yields. Thus, it has the potential to develop as a new anti-diabetic agent. This study aimed to explain the anti-diabetic effect of andrographolide compared to pioglitazone (positive control) on glucose uptake, PPARγ, and GLUT-4 mRNA levels in 3T3-LI Swiss albino adipocytes, an in vitro model used in the study of adipocytes biology. In the study, the mature adipocyte cells were differentiated from 3T3-L1 fibroblasts by incubation with 3-isobutyl-1-methylxanthine, dexamethasone, and insulin. Andrographolide was provided through direct isolation from the A. paniculata herbs. The gene expression was detected using the reverse transcription polymerase chain reaction method. We found that pioglitazone and andrographolide significantly increased glucose uptake capability. Andrographolide was able to increase PPARγ and GLUT-4 mRNA levels compared to pioglitazone with the best concentration at 5.6 µM of andrographolide. In conclusion, andrographolide was demonstrated to improve glucose uptake by increasing PPARγ and GLUT-4 mRNA levels; thus, it has the potential to develop as an antidiabetic therapeutic agent. |
