Aloe vera stimulate cell proliferation, cell migration, expression of vascular endothelial growth factor-A (VEGF-A), and c-Jun N-terminal kinase-1 (JNK-1) on fibroblast of diabetic rat models
Penulis/Author
INSANUL FIRDAUS (1); dr. Nur Arfian, Ph.D. (2); Prof. Dr. Mae Sri Hartati Wahyuningsih, Apt., M.Si. (3); Prof. Dr. dr. Denny Agustiningsih, M.Kes., AIFM. (4)
Tanggal/Date
2019
Kata Kunci/Keyword
Abstrak/Abstract
of vascular endothelial growth factor-A (VEGF-A) and c-Jun N-terminal kinase-1
(JNK-1) are important factors in wound healing process. Aloe vera contains
active compounds which can help in the wound healing process. Thestudy
aimed to investigate the effect of ethanol extract ofA. veraon cell proliferation,
cell migration, VEGF-A and JNK-1 expression of skin fibroblast cells of diabetic
rats. The primary skin fibroblast cells were isolated from diabetic Wistar rat and
incubated with the A. vera extract in various concentrations i.e. 500 (AV500),
250 (AV250), and 125 ?g/Ml (AV125) for 24, 48 and 72 h.The cell proliferation
was examined visually by counting the cells number, the cell migration was
observed using in vitro scratch assay, whereas VEGF-A and JNK-1 expression
were examined using RT-PCR. In 24 and 48h incubation,the cell proliferation
of AV500 and AV250 groups had higher number of cells than negative control
group,but there was no significant difference (p>0.05). However in72 h
incubation,the cell proliferation of AV500 group (29.33±1.28x104 cells/mL)was
significantly different compared to negative control group (22.91±3.21x104
cells/mL) (p<0>0.05).The expression of VEGF-A and JNK-1 after incubation with the AV500
for 48 h, weresignificantly higher than those of negative control group (p<0.05).
In conclusion, A. vera increases cell proliferation, cell migration, VEGF-A and
JNK-1 expression offibroblast cellof diabetic rat skin.
Rumpun Ilmu
Ilmu Kedokteran Dasar & Biomedis
Bahasa Asli/Original Language
English
Level
Nasional
Status
Dokumen Karya
No
Judul
Tipe Dokumen
Aksi
1
89_ 2019 Firdaus-Mae_compressed.pdf
Bukti Published
2
Sertifikat Akreditasi_Berkala Ilmu Kedokteran (Journal of the Medical Science).pdf
3
Full document - Aloe vera stimulate cell proliferation, cell migration,.pdf
