Alginate from Sargassum siliquosum Simultaneously Stimulates Innate Immunity, Upregulates Immune Genes, and Enhances Resistance of Pacific White Shrimp (Litopenaeus vannamei) Against White Spot Syndrome Virus (WSSV)
Penulis/Author
ERVIA YUDIATI (1); Prof. Dr. Ir. Alim Isnansetyo, M.Sc. (2); Prof. Dr. Ir. Murwantoko, M.Si. (3); Dr. Ir. Triyanto, M.Si. (4); Christina Retna Handayani (5)
Tanggal/Date
2019
Kata Kunci/Keyword
Abstrak/Abstract
Although alginate is known as an immunostimulant in shrimp, the comprehensive and simultaneous study on its activity to
resolve the relationship of the hematological parameters, upregulation of immune-related gene expression, and resistance to pathogen has not been found in shrimp. We performed experiments to evaluate the effect and mechanism of alginate from S. siliquosum on Pacific white shrimp immune system. Hematological parameters were examined after oral administration of Na
alginate in the shrimp. White spot syndrome virus (WSSV) was injected to the shrimp at 14 days, and its copy number was
examined quantitatively (qRT-PCR). Immune-related gene expression was evaluated by qRT-PCR. Alginate increased some
hematological immune parameters of shrimp. Before WSSV infection, expression levels of Toll and lectin genes were upregulated. The lectin gene were upregulated post infection, and the Toll gene in all the treatments were downregulated, except the shrimps fed with alginate at 6.0 g kg?1 at 48 h post infection (hpi). The shrimps fed with alginate at 6.0 g kg?1 were the most resistant and gave the least WSSV copy number at 48 hpi. Resistance of shrimps fed the alginate-supplemented diets against WSSV was significantly higher compared to that of the control treatment with 56% and 10% of survival rates, respectively. Oral administration of alginate did not affect the growth and total protein plasma. At 120 h post challenge, alginate treatment at 6.0 g kg?1 exhibited the highest survival rate. It is concluded that oral administration of alginate enhanced the innate immunity by upregulating immune-related gene expression. Consequently, the enhancement of the shrimp innate immunity improves the resistance against WSSV infection.
Rumpun Ilmu
Budidaya Perikanan
Bahasa Asli/Original Language
English
Level
Internasional
Status
Dokumen Karya
No
Judul
Tipe Dokumen
Aksi
1
Yudiati 2019 Alginate from Sargassum siliquosum Simultaneously Stimulates.pdf
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Editorial Mar Biotech.pdf
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Cover Mar Biotech.pdf
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Daftar Isi Mar Biotech.pdf
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2019 AlginateFromSargassumSiliquosu.pdf
Bukti Published
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Scimago Mar Biotech.pdf
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Alginate from Sargassum siliquosum Simultaneously Stimulates Innate Immunity, Upregulates Immune Genes, and Enhances Resistance of Pacific White Shrimp (Li.pdf
