Agrobacterium tumefaciens Mediated Transformation of rolC::Hd3a-GFP in Black Rice (Oryza sativa L. cv. Cempo Ireng) to Promote Early Flowering
Penulis/Author
Prof. Dr. Yekti Asih Purwestri, S.Si., M.Si. (1); Aries Bagus Sasongko, S.Si., M.Biotech. (2)
Tanggal/Date
2016
Kata Kunci/Keyword
Abstrak/Abstract
he purpose of this study was to transform rolC::Hd3a-GFP by Agrobacterium tumefaciens on black rice (Oryza sativa L. cv. Cempo Ireng). The callus formation were induced from scutellum using two different media, 2N6 and 2,4-dichlorophenoxyacetic acid (2,4-D). PCR analysis using specific primers for Hd3a and hpt was performed to determine the stability of rolC::Hd3a-GFP construct before co-cultivation of callus and Agrobacterium tumefaciens. This study indicated that black rice callus induction on 2N6 medium responded faster than that on MS 2,4-D medium and generated friable calli for Agrobaterium-mediated transformation. The screening result showed that several calli were competent to regenerate on medium containing hygromysin and demonstrated Hd3a insertion gene.
Level
Internasional
Status
Dokumen Karya
No
Judul
Tipe Dokumen
Aksi
1
22 - Similarity - 3% - Agrobacterium Tumefaciens Mediated Transformation of rolC__Hd3a-GFP in Black Rice (Oryza Sativa L_ cv_ Cempo Ireng) to Promote Early Flowering.pdf
