Activity of Noni Fruit (Morinda citrifolia L.) Ethanolic Extract on Class muGlutation S-Transferase of Lung Rat
Penulis/Author
Purwanto, M.Sc., Ph.D., Apt. (1); Prof. Dr. apt. Sudibyo Martono, M.S. (2)
Tanggal/Date
7 2020
Kata Kunci/Keyword
Abstrak/Abstract
One of the main modalities of cancer treatment is chemotherapy, which uses chemicals
that are generally electrophilic. These xenobiotic compounds sometimes does not produce
effective response due to activity of glutathione S-transferase (GST) which inactivate the
xenobiotics. Several natural phenolic compounds were reported to inhibit GST activity
in vitro. Noni fruit (Morinda citrifolia L.) which contains flavonoids and other phenolic
compounds such as scopoletin and morindon is proposed to interfere GST activity. This
study aimed to analyze the effect of ethanolic extract of Noni fruit in vivoon GST activity
in lung rat using 1,2-dichloro-4-nitrobenzene (DCNB). This substrate is a specific for class
mu GST. First, rats were administered with ethanolic extract of Noni and dimethylbenz(?)
anthracene (DMBA) for two weeks. The cytosolic fraction of lung was isolated then the GST
activity was determined by simple kinetic program which was automatically calculated
using spectrophotometer. The results showed that ethanolic extract of Noni in 1 and 5%
(w/v) of concentration induced class muGST activity, whereas 10% (w/v) of concentration
inhibited class muGST activity. After a treatment with DMBA, all tested concentrations of
ethanolic extract of Noni inhibited class mu GST activity of lung rat significantly. These
results indicated that Noni fruit extract can be further developed as a supportive agent of
a chemotherapy drug.
Rumpun Ilmu
Analisis Farmasi dan Kimia Medisinal
Bahasa Asli/Original Language
English
Level
Nasional
Status
Dokumen Karya
No
Judul
Tipe Dokumen
Aksi
1
Purwanto and Sudibyo, 2020, Activity of Noni fruit.pdf
