Karya
Judul/Title Activities of Plant Cell Wall Degrading Enzymes by Bacterial Soft-rot of Orchid
Penulis/Author Prof. Dr. Tri Joko, S.P., M.Sc. (1) ; AHMAD SUBANDI (2); RR NANDA KUSUMANDARI (3); Dr. Ir. Arif Wibowo, M.Agr.Sc. (4); Prof. Ir. Achmadi Priyatmojo, M.Sc., Ph.D. (5)
Tanggal/Date 2014
Kata Kunci/Keyword
Abstrak/Abstract Soft rot by bacterial pathogens is one of the most widespread and destructive diseases on various plants including orchids throughout the world. The pathogenicity of the pathogens is reported to be mainly determined by massive production of plant cell wall-degrading enzymes (PCDE). In the previous work, we have isolated 20 isolates of bacterial soft rot from orchids collected in Yogyakarta Special Region and West Java province, Indonesia. In this study, we further confirmed them as pathogens by hypersensitive reaction assay on tobacco leaves followed by pathogenicity test on Phalaenopsis sp. The production of four major PCDE by qualitative plate assays including pectate lyase, polygalacturonase, cellulase and protease was also evaluated. Even though all the isolates were able to initiate soft rot symptom, our results showed two distinct groups which clustered as producing and non-producing PCDE. The 16S rDNA analysis revealed that the isolates belonged to the genera Pectobacterium, Klebsiella, Serratia, Enterobacter, Citrobacter, Providencia and Pseudomonas.
Rumpun Ilmu Ilmu Hama dan Penyakit Tanaman
Bahasa Asli/Original Language English
Level Internasional
Status
Dokumen Karya
No Judul Tipe Dokumen Aksi
1TriJoko-print edition.pdf
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