Active Fractions of Black Rice Bran cv Cempo Ireng Inducing Apoptosis and S-phase Cell Cycle Arrest in T47D Breast Cancer Cells
Penulis/Author
Prof. Dr. Rarastoeti Pratiwi, M.Sc. (1); ALFI RIZQI AMALIA (2); Woro Anindito Sri Tunjung, S.Si., M.Sc., Ph.D. (3); apt. Rumiyati, S.Si., M.Si., Ph.D. (4)
Tanggal/Date
2019
Kata Kunci/Keyword
Abstrak/Abstract
Abstract. Indonesian black rice cv Cempo Ireng was evaluated for cancer
prevention using a T47D cell line model. Methanolic extract of black rice bran
(BRB) showed cytotoxicity with an IC50 of 522.13 µg/ml. This result was in
contrast to water extract of BRB, which yielded an IC50 of more than 6000
µg/ml. From the methanolic extract of BRB, its 6 fractions were found from the
preparative TLC performance, with Rf values ranging from 0 to 0.97. According
to the MTT assay of all fractions, fraction 3 (F3) had the lowest IC50 (60.17 ±
1.72µg/ml), while fraction 2 (F2) had an IC50 of 64.3 ± 0.61µg/ml. However, F2
showed the highest induction of apoptosis, i.e. about 75.39 ± 0.43% compared to
the T47D cells without treatment (control) (about 8.39 ± 0.16%) and with
doxorubicin treatment (about 41.30 ± 0.08%). Furthermore, fraction 5 (F5) not
only induced apoptosis but also increased the S-phase arrest percentage (60.36 ±
2.07%), which was significantly higher than for the other treatments. Therefore,
each fraction of BRB showed different cytotoxic responses to T47D cells.
Keywords: apoptosis; cell cycle; Cempo Ireng; cytotoxicity; S-phase.
Rumpun Ilmu
Biologi (dan Bioteknologi Umum)
Bahasa Asli/Original Language
English
Level
Internasional
Status
Dokumen Karya
No
Judul
Tipe Dokumen
Aksi
1
PC_Rarastoeti_Active Fractions of Black Rice Bran cv Cempo Ireng Inducing Apoptosis and S-phase Cell Cycle Arrest in T47D Breast Cancer Cells.pdf
[PAK] Cek Similarity
2
SK_Active Fractions of Black Rice Bran cv Cempo Ireng Inducing.pdf
Dokumen Pendukung Karya Ilmiah (Hibah, Publikasi, Penelitian, Pengabdian)
3
Full Dokumen_Active Fraction_.pdf
[PAK] Full Dokumen
4
BKM JMFS S-phase.pdf
[PAK] Bukti Korespondensi Penulis
5
Active Fractions of Black Rice Bran cv Cempo Ireng.pdf
[PAK] Full Dokumen
6
BK_JMFS_Active Fractions of Black Rice Bran cv Cempo Ireng (1).pdf
