Active cutaneous anaphylaxis-inhibitory activity of Gamavuton-0 potassium (K-GVT-0)
Penulis/Author
Prof. Dr. apt. Agung Endro Nugroho, S.Si., M.Si. (1); Dr. apt. Nunung Yuniarti, S.F., M.Si. (2); Enade Perdana Istyastono (3); Prof. Dr. Supardjan AM, M.S., Apt. (4); Prof. Dr. Lukman Hakim, M.Sc., Apt. (5)
Tanggal/Date
2007
Kata Kunci/Keyword
Abstrak/Abstract
Potassium Gamavuton-0 is a salt form of 1,5-bis(4’-hydroxy-3’-
methoxyphenyl)-1,6-heptadiene-3,5-dione or gamavuton-0. Structure
modification to a salt form is expected to result in a soluble compound which
should have a better bioavailability. The purpose of this experimental study
was to evaluate the inhibitory effects of potassium gamavuton-0 on active
cutaneous anaphylaxis reaction induced by ovalbumin as an antigen.
The study was performed using an active cutaneous anaphylaxis
method followed by histopathological observation. The rats were
subcutaneously sensitised by ovalbumin (OVA) and Al(OH)3 twice on the days
of 0 and 7, and finally were challenged by ovalbumin on the day of 14 to
induce active cutaneous anaphylaxis reaction. In order to determine the mast cells on the inflammation tissues, the specimens were stained with 0.1 %
Alcian Blue (pH 0.3) and nuclear fast red. Under light microscopy, mast cells
were identified as blue granules against a pale red backgound.
The results showed that potassium gamavuton-0 at the doses of 20
and 40 mg/kg BW could inhibit the pigmentation area of vascular
permeability on rats dorsal skin. It indicated that this compound has the
inhibitory effect on active cutaneous anaphylaxis reaction with ED30 value of
28.26 mg/kg BW. Nevertheless, inhibitory effect of potassium gamavuton-0
was still lower than that of cromolyn sodium, a mast cell degranulation
inhibitory drug. Histopathologically, the mechanism of inhibitory effect of
potassium gamavuton-0 on active cutaneous anaphylaxis reaction is
suggested through the inhibition on mast cell degranulation process
responsible for releasing inflammation mediators such as histamine,
serotonin.
Rumpun Ilmu
Farmakologi dan Farmasi Klinik
Bahasa Asli/Original Language
Bahasa Indonesia
Level
Nasional
Status
Dokumen Karya
No
Judul
Tipe Dokumen
Aksi
1
Active cutaneous anaphylaxis-inhibitory activity of KGVT-0.pdf
