| Abstrak/Abstract |
Objective: This study aimed to observe the effects of Achatina fulica mucus (AFM) on ultraviolet B (UVB)-induced fibroblast photoaging by assessing
monocyte chemotactic protein (MCP)-1, vascular endothelial growth factor (VEGF), matrix metalloproteinases (MMP)-3, and MMP-12 mRNA
expressions.
Methods: Cell cultures of normal human dermal fibroblasts (NHDFs) were divided into six groups: Group 1 was normal fibroblasts without UVB
irradiation as normal control and Groups 2–5 consisted of 100 mJ/cm2 UVB-induced aged fibroblasts. Group 2 had no treatment as negative control,
Group 3 was treated by platelet-rich plasma 10% as positive control group, and Groups 4–6 were treated by various concentrations of AFM (3.9 ?l,
15.625 ?l, and 62.5 ?l). The MCP-1, VEGF, MMP-3, and MMP-12 mRNA expressions in the different NHDF groups were assessed by quantitative
polymerase chain reaction.
Results: The mRNA expressions of MCP-1, VEGF, MMP-3, and MMP-12 in the AFM group compared to the UVB group decreased 8, 5, 5, and 4 folds,
respectively. AF62 exhibited the highest improvement among the other AFM-treated groups.
Conclusion: AFM treatment attenuates UVB-induced fibroblasts photoaging by reducing inflammation, angiogenesis, and matrix metalloproteinases. |
