Accuracy of Estrus Detection Through Vaginal pH in Saanen Ettawah Crossbreed Goat
Penulis/Author
Prof. Ir. Diah Tri Widayati, MP., Ph.D., IPM (1); PRADITA IUSTITIA S (2); Dr. Ir. Sigit Bintara, M.Si., IPU., ASEAN Eng. (3); Prof. Dr. Ir. Budi Prasetyo Widyobroto, DESS., DEA., IPU., ASEAN Eng. (4)
Tanggal/Date
2018
Kata Kunci/Keyword
Abstrak/Abstract
Weak estrus behavior potentially to be cause of a decline in reproductive efficiency in goat.
The present study investigated the effect of measuring the vaginal pH on the detection of
estrous in Saanen Ettawah crossbreed goat. Furthermore, the vaginal pH is confirmed with
estrogen profile in each estrous phase. For this purpose, 13 Saanen Ettawah cross breed does
(Capra hircus) with BCS 2-3 were used. The does were feed concentrate, ad libitum forage
and water. The experiment was conducted with during the natural estrous cycle. Vaginal smears
were collected from each does at every day over a 60-day period to determine the timing of
each phase of the estrous cycle. The vaginal pH measurements were carried out together with
blood sampling. Measurements of the pH of the vaginal mucus is done with paper pH indicator
The blood were collected from the caudal vein into anticoagulant-coated tubes following
estrous cycle, covers estrus (E/day-0), metestrus (M/day-3), diestrus (D/day-12) and proestrus
(P/day-15). The concentration of plasma estrogen was determined using a solid phase
competitive Enzyme-Linked Immune Sorbent Assay (ELISA, DRG, Germany). We found
linkages between vaginal pH with each phase of estrous cycle and estrogen. The highest level
of vaginal pH and estrogen were found on estrus. The lowest vaginal pH was observed in
diestrus, whereas the lowest estrogen were found in diestrus. It was concluded that vaginal
pH related to the estrous phase, indicating that vaginal pH could be a useful parameter for
estrus detecting.
