A rapid and simple high-performance liquid chromatographic method for determination of levofloxacin in human plasma
Penulis/Author
Prof. Dr. apt. Sudibyo Martono, M.S. (1); Prof. Dr. apt. Zullies Ikawati. (2); apt. Arief Rahman Hakim, S.Si., M.Si. (3); Prof. Dr.rer.nat. apt. Raden Rara Endang Lukitaningsih, S.Si., M.Si. (4)
Tanggal/Date
2017
Kata Kunci/Keyword
Abstrak/Abstract
A simple and rapid high-performance liquid chromatography method was developed and validated for quantifying LEV in human plasma. Chromatographic separation was performed under isocratic elution on a Luna Phenomenex® C18 (150 × 4.6 mm, 5 µm) column. The mobile phase was comprised of acetonitrile, methanol, and phosphate buffer 25 mM pH 3.0 (13:7:80 v/v/v) and pumped at a flow rate of 1.5 mL/min. Detection was performed by UV detector at a wavelength of 280 nm. Samples were pre-treated with acetonitrile followed by centrifugation, evaporation, and reconstitution step. The method proved linear (r = 0.995), sensitive (LLOQ and LOD was 1.8 and 0.6 µg/mL respectively), accurate (% error above LLOQ ? 12% and LLOQ ? 20%), precise (RSD ? 9%), robust in the ranges of 1.8-28.8 µg/mL, rapid (separation time not more than 10 min), and simple (use no organic additive in mobile phase). The method was showed reliable for quantifying LEV in human plasma.
Rumpun Ilmu
Farmasi Lain Yang Belum Tercantum
Bahasa Asli/Original Language
English
Level
Internasional
Status
Dokumen Karya
No
Judul
Tipe Dokumen
Aksi
1
25_ Naskah IJC-17-1-2017.pdf
[PAK] Full Dokumen
2
25_ Bukti Korespondensi IJC-17-1-2017.pdf
[PAK] Bukti Korespondensi Penulis
3
EDITORIAL BOARD.pdf
[PAK] Halaman Editorial
4
Vol 17, No 1 (2017)-Table of contents.pdf
[PAK] Daftar Isi
5
A Rapid and Simple High-Performance Liquid Chromatographic Method for Determination of Levofloxacin in Human Plasma.pdf
