Abstrak/Abstract |
Bengkuang (Pachyrhizus erosus) has been traditionally used as sun screening
and skin whitening. The active compounds in bengkuang extract already published
included their activities in antioxidant and skin whitening. However, standardization of
bengkuang extract has not been studied. Therefore, current research was conducted
to find out the analysis procedure by High Performance Liquid Chromatography to
make standardization bengkuang extract. The first step of this research was collecting
bengkuang from Prembun, Central Java, Indonesia in dry season. After cleaning and
peeling, bengkuang root was sliced, dried and ground to make powder. Then followed
by extraction using Soxhlet in petroleum ether and subsequently in methanol. Methanol
extract was evaporated and then partitioned with ethyl acetate-water. Ethyl acetate
fraction was evaporated and then separated in open column chromatography using silica
gel as stationary phase and a gradient mixture of chloroform-ethyl acetate-methanol as
mobile phase. Bio guided fraction method was used for separation and purification to
get isolated compounds. The isolated compounds obtained from this fractionation were
then elucidated and analyzed their activities.
A new compound (8,9-furanyl-pterocarpan-ol) has been selected as a biomarker for
extract standardization. The optimum of HPLC condition for standardization consisted
of a column (Zorbax SB-C18; i.d. 0.46 cm; 5 ?m particle size), mobile phase (gradient
elution of MeOH-water) with flow rate of 1 mL/min and detector (UV-detector at 293
nm). The obtained LOD value was 0.51 ± 0.02 ?g. The potentials of this compound to
absorb UV ray, antioxidant and anti-tyrosinase were 4.018 mAU*S/mmL; 2.113±0.001mM
(SC50); 7.19±0.11 mM (IC50), respectively. |