2,2'-diphenil-1-picrylhydrazil (DPPH) radical scavenging activity of extracts and fractions of rambutan (Nephelium lappaceum L.) peel
Penulis/Author
Prof. Dr. Abdul Rohman, S.F., M.Si., Apt. (1)
Tanggal/Date
2016
Kata Kunci/Keyword
Abstrak/Abstract
Objectives: Rambutan, Nephelium lappaceum L. is tropical fruit widely distributed in South-East Asia. The consumption of rambutan fruit results in the production of vast amounts of waste from peels of the fruit. Methodology: This study is intended to evaluate the radical activity of extracts and fractions of rambutan peel cultivar simacan and lebak bolus toward 2,2'-diphenil-1-picrylhydrazil radical (DPPH) and to correlate its activity with total phenolics and flavonoid contents. Rambutan peel is dried, powdered and macerated using methanol to obtain methanolic extract. The methanolic extract was further subjected to fractionation using petroleum ether and ethyl acetate. The extracts and fractions were evaluated for its radical scavenging activity as well as for determination of phenolics and flavonoids contents. Results: Among extracts and fractions evaluated, ethyl acetate fraction of rambutan peel cultivar lebak bolus has the most active antiradical activity with IC50 value of 2.732 ?g mL-1. Quercetin was used as positive control with IC50 of 1.998 ?g mL-1. The ethyl acetate fraction of rambutan peel cultivar lebak bolus also has the highest phenolics and flavonoid contents, accounting of 47.71% (wt/wt) gallic acid equivalent and 29.59% (wt/wt) rutin equivalent, respectively. The correlation between IC50 and phenolics content revealed R2 of 0.594, while R2 for the relationship between IC50 and flavonoid content is 0.323, meaning that phenolics and flavonoid contents contributed to 59.4 and 32.30% of antiradical scavenging activity, respectively. Conclusion: Extract of rambutan peel is potential to be used as antiradical.
